酵母不對(duì)稱(chēng)還原4-羥基-2-丁酮為(R)-1，3-丁二醇的初步研究.pdf

1、江南大學(xué)碩士學(xué)位論文酵母不對(duì)稱(chēng)還原4羥基2丁酮為（R）1，3丁二醇的初步研究姓名：楊套偉申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：微生物學(xué)指導(dǎo)教師：饒志明20090601AbstractAbstractChiralcompoundshavebeenwidelyappliedinpharmaceuticals，food，pesticideandchemicalindustryduetotheirspecialopticalactivityWiththepr

2、ogressinpharmacologyofchiralpharmaceuticalsandthestrictregulationontheapplicationofnewracemicdruginrecentyears，theresearchanddevelopmentofchiralpharmaceuticalshavebecomethenewfocusanddirectionEnantiomericallypurealcohols

3、areimportantintermediatesforsynthesisofpharmaceuticalsAsymmetricreductionprochiralketonescatalyzedbymicroorganismortheoxidoreductaseinthecellhasbeenanimportantmethodofsynthesisenantiomericallypurealcohols(R)I，3butanediol

4、((R)I，3BDO)isanimportantmaterialforsynthesizingvariousopticallyactivecompoundssuch38azetidinonederivatives，whichareintermediatematerialforantibioties，pheromones，fragrancesandinsecticidesIthasreportedthatmicrobialscanbeus

5、edteducecarbonylcompoundsintothechiralalcoh01Inthiswork，thereductionofreactionsof4hydroxy2butanone(4H2B)intochlorine13butanediolcatalyzedbyyeastceilswillbestudiedThemaincontentsofthisworkareasfollows：(1)HBY61wasscreenedf

6、rom80yeaststrainspreservedinourlaboratory，whichshowedahi曲bioreductionactivityandenantioselectivityTheproductofasymmetricreductionwasenantiomericallypure(R)1，3BDOTheidentitiesofthestrainswereestablishedbasedontheir18SrRNA

7、sequencesandphysiologicalcharacteristicsResultsindicatedthestrainwhichdesignatedasHBY61wasavarietyofPichiajadiniiwithsimilaritiesof997％(2)Thesampleofsubstrateandproductwereanalyzedqualitativelycomparingwithgaschromatogra

8、phicchartofstandard，theexternalstandardmethodwasusedtodeterminetheirquantityofsample(3)TheexperimentwasusedtooptimizeparametersofthebioreductionconditionofyeastCompositionofculturemediawasdiscussed，theanalyticalresultsin

9、dicatedthattherearethreefactorsincludingglcose，tryponeandK2HP04whichhaveremarkableeffectonbiomassofyeastBasedontheexperiment，themaximalyieldofthebiomass(151g／L)couldbeobtainedwhentheconcentrationsofglcose，yeastextract，tr

10、yptone，KH2P04andMgS04weresetat120g／L，20g／L，20g／L，5g／Land075g／L，respectivelyattheoptimalcultivationnaturalpH，rotatespeed200r／min，inoculationsize2％Inthisconditionthebiomassis19timesoftheinitializationThe(R)1，3BDO(58g／L)and

11、thebiomass(152g／L)wereachievedinstandardreactionsystemusingtheoptimizedmediumThegrowthcurveofyeastwasdetected，whichshowedthehighactivityyeastcouldbeobtainedfor2628hr(4)Thefactorshavingeffectonasymmetricbioreduction4H2Bby

12、yeastHBY61hadbeeninvestigatedinstandardreactionsystemThetechnicalconditionsofbioreductionbyyeastHBY61wereobtained，includingappropriatetemperature(30℃)，PH(7o)，initialsubconcentration(1％)，carbonresource，andglcose(6％)assoon