扶正抗癌方對(duì)H-,22-腹水型瘤株移植瘤小鼠Survivin和NF-κB表達(dá)影響的實(shí)驗(yàn)研究.pdf

1、陜西中醫(yī)學(xué)院碩士學(xué)位論文扶正抗癌方對(duì)H腹水型瘤株移植瘤小鼠Survivin和NF-κB表達(dá)影響的實(shí)驗(yàn)研究姓名：李秋薦申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：中西醫(yī)結(jié)合臨床指導(dǎo)教師：陳光偉20100401To Study the Improvement Function of Fu Zheng KangAiFang to H22 hepatica of the level of Survivin and NF-κB Survivin and NF-κB

2、in H22 hepatica tumor Cell Cytoplasm Abstract Objective: To study the resistant function of Fu Zheng KangAiFang to H22 hepatica to the weight ,the rate of restraining tumor, the index of immune organs (thymus

3、, spleen),the level of Survivin and NF-κB in H22 hepatica tumor cell cytoplasm of BALB/C. Discusses its anti-tumor the immunity mechanism and to provide the scientific basis for anti-tumor treatment of the TCM on clinica

4、l. Methods: 50 mouse of BALB/C were divided complete randomizing into 5 group and each group of 10.Respectively is: the blank group control group (A group), tumor-bearing control group (B group), the tegafur

5、control group(C group), Fu Zheng KangAiFang big monitoring group (D group), Fu Zheng KangAiFang small the monitoring group (E group).H22 hepatica cells were transplanted subcutaneously to BALB/C mouse withright maxillary

6、 on the sterilized worktable.A group(0.9%NS,90mg/kg);Bgroup(0.9%NS,90mg/ kg);Cgroup(tegafur 25mg/kg 0.2ml);D,E,group(respectively by 2.28g/kg/d, 0.57g/kg/d,0.2ml) giving medicine, A、B、D、E group, from the first to the

7、 fourth day. C group from the 1-5th day. Observation feed, thinking water, active, state of mind, and so on. Make the recode everyday and the body weight , killing mouse by severing the jugular to obtain thymus, spleen,

8、 transplanting tumor, measuring the weight curve, calculating the general living conditions of each mouse, examining the level of Survivin and NF-κB in H22 hepatica tumor cell cytoplasm. Result: Compare