

版權說明:本文檔由用戶提供并上傳,收益歸屬內容提供方,若內容存在侵權,請進行舉報或認領
文檔簡介
1、The role of IL-23/IL-17 Axis in Cardiac Allografts rejection in Human and Mice.
Part Ⅰ
Dynamic changes in IL-23/IL-17 Axis in patients with acute cellular rejection after cardiac transplantation.
Objective
2、: To study the role of IL-23/IL-17 Axis in the acute rejection after cardiac transplantation.
Methods: In a cross-sectional study, peripheral blood and endomyocardial biopsies (EMB) of 17 heart transplant recipients
3、 were analyzed.Patients were divided in two groups: stable and acute rejection (AR).The gene expressions including T-bet, IFN-γ, RORγt, IL-17, IL-23, GATA3, and FoxP3, the functional marker of Th1, Th17, Th2, and FoxP3+
4、CD4+ T cells were quantified at the mRNA or protein level in combination with the cell profiles.And then, these CD4+ T-cell subsets in peripheral blood and endomyocardial biopsies (EMB) in patients with stable-graft and
5、acute cellular rejection was analysis.
Results: We observed that the IL-23/IL-17 axis related gene expressions including T-bet, IFN-γ, RORγt, IL-17 and IL-23, were elevated in EMB samples from patients with acute gr
6、aft rejection.Accordingly, the percentages of circulating Th1, Th17, and FoxP3+ CD4+ T cells were also significantly increased.
Conclusions: The data suggest that IL-23/IL-17 axis and -related CD4+ T cells are assoc
7、iated with acute graft rejection in patients with cardiac transplantation.
Part Ⅱ
The experimental study on antagonist IL-23/17 Axis to attenuate chronic rejection of cardiac allograft in mice.
Objective:
8、To study the effect and mechanism of the antagonistic anti-p40 antibody on chronic cardiac rejection.
Methods: Hearts of the B6.C-H2bm12/KhEg mice were transplanted into MHC class Ⅱ-mismatched C57Bl/6J mice (Wild Ty
9、pe, γδTCR -/- and IL-17-/-).It is an established murine model of chronic allograft rejection without immunosuppression.The mice were treated with control IgG or 200 μg anti-p40 monoclonal antibody on postoperative days,
10、respectively.Abdominal palpation and echocardiography were used to monitor the survival of the grafts.The immunohistochemical method, flow cytometry and Quantitative real-time polymerase chain reactiona (Q-PCR) were used
11、 to study the expression of inflammation cytokines and immunity cells.
Results: The mice administrated with anti-p40 antibody showed a significant promotion in graft survival (median survival time > 100 d), and hist
12、ological analyses revealed that the cardiac allograft rejection was attenuated.Q-PCR and immunofluorescence analyses demonstrated that anti-p40 antibody downregulated the level of ingraft cytokine and chemokine expressio
13、n (IL-6, IFN-γ, IL-17a, CCL2 and CCL20).Flow cytometry analyses shown that γδ T cells was important ingraft source of IFN-γ and IL-17a and inhibited to produce inflammation cytokine by anti-p40 antibody.Compared with the
溫馨提示
- 1. 本站所有資源如無特殊說明,都需要本地電腦安裝OFFICE2007和PDF閱讀器。圖紙軟件為CAD,CAXA,PROE,UG,SolidWorks等.壓縮文件請下載最新的WinRAR軟件解壓。
- 2. 本站的文檔不包含任何第三方提供的附件圖紙等,如果需要附件,請聯(lián)系上傳者。文件的所有權益歸上傳用戶所有。
- 3. 本站RAR壓縮包中若帶圖紙,網(wǎng)頁內容里面會有圖紙預覽,若沒有圖紙預覽就沒有圖紙。
- 4. 未經權益所有人同意不得將文件中的內容挪作商業(yè)或盈利用途。
- 5. 眾賞文庫僅提供信息存儲空間,僅對用戶上傳內容的表現(xiàn)方式做保護處理,對用戶上傳分享的文檔內容本身不做任何修改或編輯,并不能對任何下載內容負責。
- 6. 下載文件中如有侵權或不適當內容,請與我們聯(lián)系,我們立即糾正。
- 7. 本站不保證下載資源的準確性、安全性和完整性, 同時也不承擔用戶因使用這些下載資源對自己和他人造成任何形式的傷害或損失。
最新文檔
- 從IL-23-IL-17炎癥軸探討強的松對母胎免疫耐受的分子機制.pdf
- IL-23、IL-17及IL-23-IL-17與潰瘍性結腸炎發(fā)病機制的研究.pdf
- 潰結靈對UC大鼠IL-23-IL-17炎癥軸的調節(jié)機制研究.pdf
- IL-23-IL-17炎癥軸在實驗性自身免疫性肝炎中的作用.pdf
- 過敏性紫癜患者IL-23-IL-17軸相關因子的檢測.pdf
- IL-23-IL-17軸在EAE發(fā)病機制中的作用及其實驗性干預研究.pdf
- IL-23、IL-17與移植免疫的相關性研究.pdf
- 高分辨率熔解曲線分析技術探討炎癥免疫反應“IL-23-IL-17”軸與大腸腫瘤關聯(lián)性研究.pdf
- IL-17在大鼠心臟移植急性排斥反應中的動態(tài)觀察及意義研究.pdf
- IL-23-IL-17在橋本甲狀腺炎甲狀腺組織中的表達研究.pdf
- MiR155在心臟移植物排斥反應的作用及機制研究.pdf
- SOCS3在心臟移植慢性排斥反應中的作用和機制研究.pdf
- 狼瘡性腎炎患者血清IL-23-IL-17炎癥軸及TL1A水平變化及其意義.pdf
- IL-17和IL-21在腎移植受者中的表達及其與排斥反應的關系.pdf
- IL-17及IL-22在大鼠肝移植急性排斥反應中表達的動態(tài)觀察及意義.pdf
- IL-23-IL-17炎癥軸在非壓迫性椎間盤髓核突出致神經根損傷中的表達及意義的研究.pdf
- IL-17在大鼠肝移植急性排斥反應模型中的表達和作用研究.pdf
- MicroRNA499過表達在心臟移植慢性排斥反應中的作用及機制研究.pdf
- 質粒載體轉導的IL-10基因治療對小鼠心臟移植免疫排斥反應的抑制作用研究.pdf
- 1,25-二羥維生素D3對IgA腎病大鼠中IL-23-IL-17軸的調節(jié)作用.pdf
評論
0/150
提交評論