沙棘葉黃酮的提取、抗氧化性及抗菌性的研究.pdf

1、中國農(nóng)業(yè)大學(xué)碩士學(xué)位論文沙棘葉黃酮的提取、抗氧化性及抗菌性的研究姓名：焦翔申請學(xué)位級別：碩士專業(yè)：糧食、油脂與植物蛋白工程指導(dǎo)教師：殷麗君程永強20070601AbstractSeabuekthorn(月劬弦咖Prhamno／desL)isafineplantwithhigh嘲nomicandecologicalvalueItexists∞abundantnaturalreso㈣withspeciesdiversityinChinaSe

2、abuckthornisrichinflavonoidshowever，thepresontinvestigation011SeabuekthomflavonoidswasmainlyfocusedOn古nitsandtheinformationOnleafflavonoids訌limitedInor；dertopromoterationaldevelopmentofthis刪uf辯，wechonsedtwodifferentSeabu

3、ckthomleaves，separatelyfromOinghaiProvince(ChinaSeabuckthom)andfromNeimenggu(BigBerrySeabuckthorn)asraWmaterials，extractedllavunoidsandcomparedphysiologicalactivitiesincludingstabilityantioxiduntandantibacterialactivitie

4、s11他maincontentsandresultsare88follows：(1)WithaviewofapplicationandsafetyinfoodindustrywaterandethanolwereusedassolventItwasshownthattheoperationbywaterextractionwasmoredifficultandlesseffective，comparedwithethanolextrac

5、tionEthanolcouldbeusedasanidealsolventforflavonoidextractionfromSeabuckthornleavesUltrasonicaideanplayasignificanteffectonpromotingextractionforbothwaterextraetiunandethanolextraction(2)ByHPLCdetermination，itWassuggested

6、thatthetotalflavonoidcontentinQinghaileaveswashigherthanthatintheNeimengguleavesAndthecontentofglycosideflavonoideinformerwasalsohigherthanthelatterwhilethecontentofflavunoidaglyconesinthelatterWashigherthanthatintheform

7、erItcouldbeassumedthattheQinghaileafwasgoodresoursefortheextractionofglycosideflavonoids，andNeimenggnleafcouldbeidealrawmaterialforaglyconaextraction(3)neflavonoidsfromSeabuckthomexhibitedgoodstabili夠t0heating，theweakaci

8、dOrneutralenvironmentandfoodadditiveaddition，whichsuggestedthattheflavonoidextractsweresuitableforapplicationinfoodandfoodadditiveindustryHoweveritshouldbestoredundertheenvironmentwithoutstronglightoxidantreductuntandalk

9、alinesubstances(4)neantioxidationactivitiesoffiavonoidextractfromSeabuckthomleaves，includingtheDPPHandABTSradicalscavengingeffectwereinvestigatedSeabuckthomextractsshowedgoodactivitiesandtheantioxidetinnabilityofextracts

10、withdifferentsolventsWasintheorderof65％ethanolextract舳％methanolextraetdistflledwaterextractSeabuckthronextractshadexhmitedgoodresistancetolipidpcmxidationandthewatexextractshowedthebestresistanceabilitywhichisclosetothea

11、ntioxidenteffectsofTBHQItWasfoundtheQinghniSeabuekthomleafextractshadbetterantioxidetionactivitythanthatformNeimenggu圓1rhcantibacterialeffectwasinvestigatedbycombininginhmitionzone，ⅦCandMBCItWaSshownthattheinhibitioneffe

12、ctof65％ethanolextractionwassignificantlybetterthanthatofwaterextractTheMICandMBCofethanolextractsforthesixtypesoffoodcontaminatedbacteriawe∞5～10mg／mLandthewaterextractsas20～40mg／mLQinghaiSeabuckthomleafextractexhibitedbe