豚鼠單側迷路切除后前庭內(nèi)側核c-fos表達.pdf

1、上海第二醫(yī)科大學碩士學位論文豚鼠單側迷路切除后前庭內(nèi)側核cfos表達姓名：顧美珍申請學位級別：碩士專業(yè)：耳鼻咽喉科學指導教師：殷善開20040401豚鼠單側謎路切除后前庭內(nèi)側核cfos表達ExpressionofcfosinmedialvestibularnucleusfollowingunilaterallabyrinthectomyinguineapigsAbstractObjective：Toinvestigatethec—fos

2、expressioninguineapigs’medialvestibularneuleus(MVN)followingunilaterallabyrinthectomy(UL)。Methods：Twenty—sevenguineapigswererandomlydividedintoninegroups，threeineachgroupAfterunilateral(right)labyrinthectomytheimmunohist

3、0109icaJstainingofcfoswasperformedFos—likeimmunoreactivity(FLI)wasanalyzedatfifteenminutesandlh、2h、6h、8h、12h、24h、2dand7dafterULResults：ResultsshowednoFosexpressioninthecontrolgroupBycontrast，F(xiàn)oswasconsistentlyinducedinth

4、eULguineapigsAsymmetricallabelingwasfoundinthebilateralmedialvestibularnucleus，withmoreFoslikeneuronsintheipsilateralMVNofthelesionedsidethaninthecontralateralMVN(P005)Thereactionbecameevident15minutespostULandwasintense

5、d12hoursafterthelesionFosexpressionpeakedearly(2h)anddeclinedprogressivelyHowever，weobservedasecondpeakofFosexpression(24h)，whichwasnotsoevidentasbeforeWestillfoundFosexpression7daysaftersurgeryConclusion：1)、ULcaninducer

6、eactivestrongFosimmunostainingintheMVN，whichisoneoftheimportantwayinwhichvestibularneuronsystemreactstooutsidestimulationBycomparingthesedatatothebehavioralrecoveryprocess，weconcludethattheearlyFosLIR(1～6h)likelyreflects

7、theactivationofneuralpathwaysinresponsetoULwhereasthedelayedFosLIR(24～48h)mightunderlinelongtermplasticchangesinvolvedintherecoveryprocess21、Thevestibularfunctionhasthegreatabilityofcompensation；3)、Theessenceofcfosandoth