乳桿菌的腸道定殖和菌群調(diào)節(jié)作用研究.pdf

1、江南大學碩士學位論文乳桿菌的腸道定殖和菌群調(diào)節(jié)作用研究姓名：徐致遠申請學位級別：碩士專業(yè)：食品科學指導教師：郭本恒20060301江南大學碩士學位論文AbstractItisclarifiedthattheprobioticwhichhaveapositiveinfuenceonthemetabolicactivityoftheintestinalfloramustcolonizetheIntestinaltractbeforethey

2、exenthebeneficialeffectsLactobadlluscaseiLC2W、LactobacillusplantarumSTIllandLactobacilluscaseiBD一11whichhavedifferentbenefitswerescannedbyTechnicalCenterofBrightDairyInthispaperalactobacilluswiththebestbiocharacterswasch

3、osen，anditscolonizationInIntestinaltractandjtseffectontheintestinalflorawereinvestigatedBycomparingthegrowcharactersandtheabilityforlongtermsurvivalinsimulatehumangastrointestinaltractthelactobacilluswiththebestbiocharac

4、tersWaschosenResultsshowedthatLactobacillusplantarumSTIIlsbio—characterswerebetterthanotheronesAfteranaerobicallyincubatedinMRSBrothat37℃，16h，theamountofST111wasmorethan109cfu／ml，aftertheculturesstoredat4“Cfor28d，theamou

5、ntofbacteriastillnotlessthan90％ItcouldsurvivalandgrewinMRSBrothwith75％(m／m)NaClor030％bileandexceedantibacterialeffectonsomepathogenicbacteriaSTIII’SresistanttosimulatehumangastrointestinaltractWasstrongerthananyotherbact

6、eria，pH25’SgastricjuiceandIntestinaljuicehavelittleeffectonthisstrainTwomethods，incubationandDenaturingGradientGelElectrophoresis，wereappliedtoassessgrIII’ScolonizationinintestinaltractofWistarratsResultsshowedthatSTIIIm

7、ostlycolonizedrats’colonandileum，theamountreachedseparately10’cfu／gandlO％fu／gonlylittlesTIIIcolonizedthejejunumandthecaecum，theamountwaslessthan10Pcfu／g，butitcouldn’tcolonizetherectumandtheduodenumST—IIIcolonizedthecolon

8、forabouttendays，theileumforsevendaysandthecaecumforonlythreedaysByincubationtheeffectsofSTIIIonthecompositionoftheintestinalmicroflorawasassessedResultsshowedST111couldjncreasetheamountoflactobacillusinfecesofratsanddecr

9、easetheamountsofClostridiumpefffingensandEntericbacilli，buthadlittleeffectonEnterococcusandBifidobacteriumSkimmilkwithlOScfu／mlST—Illhasimprovedthebalanceofintestinalmicroflora，andtheresultfuldosewas105cfu／ml，andtheadjus

10、tmentholdaboutfivedaysafterstoppingtheingestionTheresearchshowedLactobacillusplantammSTHIhadexcellentbiocharactersandcouldresisttothesimulatehumangastrointestinaltract；STIⅡmostlycolonizedthecolonandtheileum；Skimmilkwithl